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Genome editing with DNA-dependent polymerases

Genome editing with DNA-dependent polymerases

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Gene editing

Nature Biotechnology (2024)Cite this article

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Two CRISPR tools harness DNA-dependent polymerases for genome editing — and may overcome some limitations of prime editors.

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Fig. 1: Schematic of prime editors (PEs), DNA polymerase editors (DPEs) and click editors (CEs).

References

  1. Anzalone, AV et al. Nature 576149–157 (2019).

    Article  CAS  PubMed  PubMed Central  Google Scholar

  2. Liu, B. et al. Nat. Biotechnol. https://doi.org/10.1038/s41587-023-01947-w (2024).

    Article  PubMed  Google Scholar

  3. Ferreira da Silva, J. et al. Nat. Biotechnol. https://doi.org/10.1038/s41587-024-02324-x (2024).

    Article  PubMed  Google Scholar

  4. Chen, P. J. & Liu, D. R. Night. Rev. The gene. 24161–177 (2023).

    Article  CAS  PubMed  Google Scholar

  5. Nelson, JW et al. Nat. Biotechnol. 40402–410 (2022).

    Article  CAS  PubMed  Google Scholar

  6. Liu, B. et al. Nat. Biotechnol. 401388–1393 (2022).

    Article  CAS  PubMed  Google Scholar

  7. Feng, Y. et al. Protein Cell https://doi.org/10.1093/procel/pwac014 (2022).

    Article  PubMed  PubMed Central  Google Scholar

  8. Yan, J. et al. Nature 628639–647 (2024).

    Article  CAS  PubMed  PubMed Central  Google Scholar

  9. Anzalone, AV et al. Nat. Biotechnol. 40731–740 (2022).

    Article  CAS  PubMed  Google Scholar

  10. Yarnall, M. T. N. et al. Nat. Biotechnol. 41500–512 (2023).

    Article  CAS  PubMed  Google Scholar

  11. Levesque, S., Cosentino, A., Verma, A., Genovese, P. & Bauer, D. E. Nat. Biotechnol. https://doi.org/10.1038/s41587-024-02266-4 (2024).

    Article  PubMed  Google Scholar

  12. Wang, X. et al. Curr. Opin. Biomed. Eng. 28100491 (2023).

    Article  CAS  PubMed  PubMed Central  Google Scholar

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Author information

Authors and Affiliations

  1. Department of Chemistry and Biochemistry, University of California San Diego, La Jolla, CA, USA

    Quinn T. Cowan & Alexis C. Komor

Corresponding author

Correspondence to Alexis C. Komor.

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Competing interests

A.C.K. is a member of the scientific advisory board of Pairwise Plants, is an equity holder in Pairwise Plants and Beam Therapeutics, and receives royalties from Pairwise Plants, Beam Therapeutics and Editas Medicine via patents licensed from Harvard University. A.C.K.’s interests have been reviewed and approved by the University of California, San Diego in accordance with its conflict of interest policies. Q.T.C. declares no competing financial interests.

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Cowan, Q.T., Komor, A.C. Genome editing with DNA-dependent polymerases. Nat Biotechnol (2024). https://doi.org/10.1038/s41587-024-02372-3

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