editing – Tamfis

Lung and liver editing by lipid nanoparticle delivery of a stable CRISPR–Cas9 ribonucleoprotein

TamunofiniarisaOctober 17, 2024Science & Technology

Technology tamfitronics Main Clustered regularly interspaced short palindromic repeats (CRISPR)–Cas9-based genome editing1,2,3 has the potential to provide wide-ranging treatments for genetic diseases4,5,6 if safe and effective methods for delivering CRISPR-based therapeutics can be developed7,8. Although viral delivery of CRISPR genome editors is the most widely used method for in vivo cell editing9,10,11, viral vectors can […]

Genome editing with DNA-dependent polymerases

TamunofiniarisaAugust 24, 2024Science & Technology

Technology tamfitronics News & Views Published: 23 August 2024 Gene editing Nature Biotechnology (2024)Cite this article Subjects Two CRISPR tools harness DNA-dependent polymerases for genome editing — and may overcome some limitations of prime editors. This is a preview of subscription content, access via your institution Access options Access Nature and 54 other Nature Portfolio […]

Click editing enables programmable genome writing using DNA polymerases and HUH endonucleases

TamunofiniarisaJuly 23, 2024Science & Technology

Technology tamfitronics Doudna, J. A. The promise and challenge of therapeutic genome editing. Nature 578229–236 (2020). Article CAS PubMed PubMed Central Google Scholar Anzalone, A. V., Koblan, L. W. & Liu, D. R. Genome editing with CRISPR–Cas nucleases, base editors, transposases and prime editors. Nat. Biotechnol. 38824–844 (2020). Article CAS PubMed Google Scholar van Overbeek, […]

Precise in vivo RNA base editing with a wobble-enhanced circular CLUSTER guide RNA

TamunofiniarisaJuly 13, 2024Science & Technology

Technology tamfitronics Main Site-directed adenosine-to-inosine (A-to-I) RNA base editing is a very promising technology with a clear path for clinical application1,2. Hydrolytic deamination of A by enzymes of the ADAR family (adenosine deaminases acting on RNA) produces an inosine, which is biochemically interpreted as G in many cellular processes such as splicing or translation and, […]