Dark Light
CRISPR screens of tumor-infiltrating NK cells identify genetic checkpoints for CAR-NK therapy

Technology tamfitronics

  • Research Briefing
  • Published:

Nature Biotechnology (2024)Cite this article

Subjects

We use a CRISPR screening platform based on adeno-associated virus and the Sleeping Beauty transposon (AAV-SB-CRISPR) to perform in vivo CRISPR screens in primary natural killer (NK) cells across four different tumor models, and identify calcium homeostasis modulator family member 2 (CALHM2) as an NK cellular checkpoint protein.

This is a preview of subscription content, access via your institution

Access options

Access Nature and 54 other Nature Portfolio journals

Get Nature+, our best-value online-access subscription

24,99 € / 30 days

cancel any time

Subscribe to this journal

Receive 12 print issues and online access

214,86 € per year

only 17,91 € per issue

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF

Prices may be subject to local taxes which are calculated during checkout

Fig. 1: AAV-SB-CRISPR screens and single-cell RNA sequencing of tumor-infiltrating NK cells jointly identify CALHM2 as an NK cellular checkpoint.
41587 2024 2319 Fig1 HTML

References

  1. Marin, D. et al. Safety, efficacy and determinants of response of allogeneic CD19-specific CAR-NK cells in CD19+ B cell tumors: a phase 1/2 trial. Night. With. 30772–784 (2024). A recent clinical trial demonstrating the robust efficacy and superior safety of CAR-NK.

  2. Vivier, E. et al. Natural killer cell therapies. Nature 626727–736 (2024). Comprehensive review discussing the current status of NK therapy.

  3. Dong, M. B. Systematic immunotherapy target discovery using genome-scale in vivo CRISPR screens in CD8 T cells. Cell 1781189–1204 (2019). This paper reports an early in vivo CRISPR screens in primary T cells.

    Article CAS PubMed PubMed Central Google Scholar

  4. Ye, L. et al. In vivo CRISPR screening in CD8 T cells with AAV-Sleeping Beauty hybrid vectors identifies membrane targets for improving immunotherapy for glioblastoma. Nat. Biotechnol. 371302–1313 (2019). This paper demonstrates the application of an AAV-SB-CRISPR system in T cells.

    Article CAS PubMed PubMed Central Google Scholar

Download references

Additional information

Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

This is a summary of: Peng, L. et al. In vivo AAV–SB-CRISPR screens of tumor-infiltrating primary NK cells identify genetic checkpoints of CAR-NK therapy. Nat. Biotechnol. https://doi.org/10.1038/s41587-024-02282-4 (2024).

About this article

Technology tamfitronics Check for updates. Verify currency and authenticity via CrossMark

Cite this article

CRISPR screens of tumor-infiltrating NK cells identify genetic checkpoints for CAR-NK therapy. Nat Biotechnol (2024). https://doi.org/10.1038/s41587-024-02319-8

Download citation

  • Published:

  • DOI: https://doi.org/10.1038/s41587-024-02319-8

https://www.tamfitronics.com/privacy-policy/

Discover more from Tamfitronics

Subscribe now to keep reading and get access to the full archive.

Continue reading